Pancreatic ductal
adenocarcinoma (PDAC) is one of the most lethal human
malignancies, with a poor long-term prognosis, and effective therapeutic options are lacking. Observing the dynamics of the pathogenesis of pancreatic intraepithelial
neoplasia (PanIN) and PDAC in
tumor models can facilitate understanding of the molecular mechanisms involved in early PDAC. Furthermore, it can compensate for the research limitations associated with analyzing clinical specimens of late-stage PDAC. In this study, we orthotopically treated the pancreas with dimethylbenzanthracene (DMBA) combined with
caerulein in wild-type C57BL/6 J mice to induce
inflammation-related pancreatic
carcinogenesis. We observed that DMBA and
caerulein treatment induced a chronic consumptive disease, which caused a decrease in the relative body and pancreas weights, diminishing the health status of the mice and enhancing the
inflammation-related histological changes. Moreover, mid-dose and high-frequency treatment with
caerulein caused prolonged inflammatory damage to the pancreas and contributed to a permissive environment for the development of PDAC. CXCL12/CXCR4, CCL2/CCR2, and several
cytokines, such as
interleukin (IL)-1β,
IL-6, and
tumor necrosis factor (TNF)-α were upregulated in the
tumor tissue of DMBA and
caerulein-induced PDAC mice. This orthotopic mouse pancreatic
carcinogenesis model mimic human disease because it reproduces a spectrum of pathological changes observed in human PDAC, ranging from inflammatory lesions to pancreatic intraepithelial
neoplasia. Thus, this mouse model may improve the understanding of molecular mechanisms underlying the injury-
inflammation-
cancer pathway in the early stages of pancreatic
carcinogenesis.