Abstract | STUDY DESIGN: A transcriptional expression assessment of human samples. OBJECTIVE: SUMMARY OF BACKGROUND DATA: METHODS: Specimens of NP were collected from 81 patients and grouped into the degenerated disc group (undergoing discectomy and fusion with significant signs of disc degeneration) and the trauma control group (undergoing anterior vertebral body and disc excision and fusion without signs of disc degeneration). Lumbar spine magnetic resonance imaging, hematoxylin- eosin staining, and safranin O staining of sections of NP tissues were conducted to evaluate the severity of the disc degeneration in all samples. Quantitative reverse transcription polymerase chain reaction was performed to investigate the levels of mRNA expression of these genes, as well as those of aggrecan, type II collagen, and SRY-box 9 (SOX-9). Degenerated samples were also divided into groups according to Pfirrmann grading system to elucidate the association of severity of degeneration and gene transcriptional levels. We also tested the relationship between mRNA levels of these genes and clinical characteristics such as hypertension and diabetes mellitus. RESULTS: CONCLUSION: LEVEL OF EVIDENCE: N/A.
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Authors | Shuai Chen, Zhi-Jun Hu, Zhi-Jie Zhou, Xian-Feng Lin, Feng-Dong Zhao, Jian-Jun Ma, Jian-Feng Zhang, Ji-Ying Wang, An Qin, Shun-Wu Fan |
Journal | Spine
(Spine (Phila Pa 1976))
Vol. 40
Issue 16
Pg. 1252-60
(Aug 15 2015)
ISSN: 1528-1159 [Electronic] United States |
PMID | 25893345
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BASP1 protein, human
- Biomarkers
- Cadherins
- Calcium-Binding Proteins
- Cartilage Oligomeric Matrix Protein
- Extracellular Matrix Proteins
- Keratin-18
- Keratin-19
- Keratin-8
- Membrane Proteins
- Nerve Tissue Proteins
- RNA, Messenger
- Receptors, Virus
- Repressor Proteins
- Synaptosomal-Associated Protein 25
- matrix Gla protein
- neurochondrin
- poliovirus receptor
- Neuropilin-1
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Asian People
(genetics)
- Biomarkers
- Cadherins
(genetics)
- Calcium-Binding Proteins
(genetics)
- Cartilage Oligomeric Matrix Protein
(genetics)
- Case-Control Studies
- China
- Diabetes Mellitus
(genetics)
- Extracellular Matrix Proteins
(genetics)
- Female
- Humans
- Hypertension
(genetics)
- Intervertebral Disc Degeneration
(genetics, pathology)
- Keratin-18
(genetics)
- Keratin-19
(genetics)
- Keratin-8
(genetics)
- Lumbar Vertebrae
- Magnetic Resonance Imaging
- Male
- Membrane Proteins
(genetics)
- Middle Aged
- Nerve Tissue Proteins
(genetics)
- Neuropilin-1
(genetics)
- RNA, Messenger
(metabolism)
- Receptors, Virus
(genetics)
- Repressor Proteins
(genetics)
- Severity of Illness Index
- Synaptosomal-Associated Protein 25
(genetics)
- Transcription, Genetic
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