Effect of alpha lipoic acid co-administration on structural and immunohistochemical changes in subcutaneous tissue of anterior abdominal wall of adult male albino rat in response to polypropylene mesh implantation.

Abstract

Polypropylene mesh is commonly used in the treatment of abdominal hernia. Different approaches were addressed to improve their tissue integration and consequently reduce long-term complications. This study aimed to investigate the effect of alpha-lipoic acid (ALA) co-administration on structural and immunohistochemical (IHC) changes in the subcutaneous tissues of the anterior abdominal wall of the adult rat in response to polypropylene mesh implantation. Forty adult male albino rats were divided into: group I (control), group II (receiving ALA), group III (polypropylene mesh implantation) and group IV (mesh implantation + ALA co-administration). After 4 weeks, subcutaneous tissue samples were prepared for light microscopy and IHC study of CD34 as a marker for angiogenesis. In groups I and II rats, positive CD34 expression was demonstrated by IHC reaction, localized to endothelial cells lining small blood vessels. Group III showed an excess inflammatory reaction, deposition of both regular and irregularly arranged collagen fibres around mesh pores and few elastic fibres. CD34-positive was detected not only in cells lining small blood vessels but also in other cells scattered in the connective tissue indicating angiogenesis. In group IV, ALA co-administration resulted in less inflammatory reaction, regular collagen deposition, enhanced elastic fibres synthesis and a significant increase in CD34-positive cells and small blood vessels reflecting improved angiogenesis. ALA co-administration with polypropylene mesh implantation controlled the inflammatory reaction, helped regular collagen deposition, enhanced elastic fibres synthesis and improved angiogenesis in the subcutaneous tissue of anterior abdominal wall of adult albino rats, suggesting a possible role of ALA in optimizing mesh integration in subcutaneous tissue.

• Authors: Shireen A Mazroa, Samar A Asker, Waleed Asker, Mohamed Abd Ellatif
• Journal: International journal of experimental pathology (Int J Exp Pathol) Vol. 96 Issue 3 Pg. 172-82 (Jun 2015) ISSN: 1365-2613 [Electronic] England
• PMID: 25891652 (Publication Type: Journal Article)
• Copyright: © 2015 The Authors. International Journal of Experimental Pathology © 2015 International Journal of Experimental Pathology.

Chemical References

• Antigens, CD34
• Polypropylenes
• Thioctic Acid
• Collagen

Topics

• Abdominal Wall (surgery)
• Animals
• Antigens, CD34 (metabolism)
• Collagen (metabolism)
• Elastic Tissue (drug effects, metabolism, pathology)
• Equipment Design
• Foreign-Body Reaction (etiology, metabolism, pathology, prevention & control)
• Immunohistochemistry
• Inflammation (etiology, metabolism, pathology, prevention & control)
• Male
• Neovascularization, Physiologic (drug effects)
• Polypropylenes (adverse effects)
• Rats
• Subcutaneous Tissue (drug effects, metabolism, pathology, surgery)
• Surgical Mesh (adverse effects)
• Thioctic Acid (administration & dosage)
• Time Factors
• Wound Healing (drug effects)