Abstract | BACKGROUND: METHODS: RESULTS: Four of the tested uremic solutes ( p-cresol, phenol, L-tyrosine, uric acid) were effective antioxidants and their IC50 were found in three model systems: ABTS(•+), HOCl/OCl(-) and crocin bleaching assay. In the 4-solutes mixtures, each one of the solute captured 12.5% for the IC50 of the mixture to ABTS(•+) or HOCl/OCl(-), exhibiting a virtually exact additive effect. In the 2-solutes mixtures, for ROO(•) capture, it was observed the need of more mass of uremic solutes to reach an IC50 value that was higher than the projected IC50, obtained from the IC50 of single solutes (25% of each, in the binary mixtures) in the same assay. In model systems for O2(•-) and H2O2, none of the uremic solutes showed scavenging activity. CONCLUSIONS: The use of the IC50 as an analytical tool to prepare and analyze mixtures allows the determination of their scavenging capacities and may be useful for the assessment of the antioxidant status of biological samples under conditions of altered levels of the endogenous antioxidant network and/or in the employment and monitoring of exogenous antioxidant therapy.
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Authors | Renata P Assis, Juliana F A Castro, Vânia O Gutierres, Carlos A Arcaro, Renata S Brotto, Olga M M F Oliveira, Amanda M Baviera, Iguatemy L Brunetti |
Journal | BMC nephrology
(BMC Nephrol)
Vol. 16
Pg. 50
(Apr 11 2015)
ISSN: 1471-2369 [Electronic] England |
PMID | 25886160
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Cresols
- Hippurates
- Reactive Oxygen Species
- 4-cresol
- Uric Acid
- Tyrosine
- Methylguanidine
- Urea
- Arginine
- Creatinine
- hippuric acid
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Topics |
- Arginine
(metabolism)
- Biomarkers
(urine)
- Creatinine
(metabolism)
- Cresols
(metabolism)
- Hippurates
(metabolism)
- Humans
- In Vitro Techniques
- Kidney Function Tests
- Methylguanidine
(metabolism)
- Oxidative Stress
(physiology)
- Reactive Oxygen Species
(metabolism)
- Renal Dialysis
(adverse effects, methods)
- Renal Insufficiency, Chronic
(therapy, urine)
- Sensitivity and Specificity
- Severity of Illness Index
- Tyrosine
(metabolism)
- Urea
(metabolism)
- Uric Acid
(metabolism)
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