[Thiamine diphosphate pool and its biosynthesis in the liver in galactosamine hepatitis].

Abstract

The hepatitis-like changes were induced in the liver of albino female rats weighing 120-150 g and fed on the appropriate vivarium diet by single parenteral administration of hydrochloride galactosamine in a dose of 0.9 or 1.8 mmol per 1 kg of body weight. The thiamine diphosphate level in the cytosol fraction of the liver decreased 24 h after the preparation administration, the same in blood but with the higher dose used. The activity of pyruvate dehydrogenase, a thiamine diphosphate dependent enzyme, decreased similarly. The cytosol transketolase activity lowered by 38-39%. The coenzyme biosynthesis disturbance due to a fall by 49-58% in the thiamine pyrophosphatase activity is considered to be responsible for hydrochloride galactosamine-induced decrease in the thiamine diphosphate pool. Specificity of the thiamine diphosphate pool disturbance and discoordination of thiamine diphosphate dependent enzymes in the liver are observed under administration of hydrochloride galactosamine.

• Authors: R V Trebukhina, A G Moiseenok, V G Petushok, V N Tumanov, B I Gorenshteĭn, V M Sheĭbak, S N Omel'ianchik
• Journal: Ukrainskii biokhimicheskii zhurnal (1978) (Ukr Biokhim Zh (1978)) 1989 Jul-Aug Vol. 61 Issue 4 Pg. 73-7 ISSN: 0201-8470 [Print] Ukraine
• Vernacular Title: Pul tiamindifosfata i ego biosintez v pecheni pri galaktozaminovom gepatite.
• PMID: 2588337 (Publication Type: Journal Article)

Chemical References

• Multienzyme Complexes
• Galactosamine
• Ketone Oxidoreductases
• 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
• Transketolase
• Thiamine Pyrophosphate

Topics

• 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
• Animals
• Chemical and Drug Induced Liver Injury (enzymology)
• Female
• Galactosamine (toxicity)
• Ketone Oxidoreductases (metabolism)
• Mitochondria, Liver (enzymology)
• Multienzyme Complexes (metabolism)
• Rats
• Thiamine Pyrophosphate (biosynthesis)
• Transketolase (metabolism)