Nonlethal dose of silver nanoparticles attenuates TNF-α-induced hepatic epithelial cell death through HSP70 overexpression.

Abstract

Silver nanoparticles (Ag-nps) have been widely used in various biomedical products. Compared with its hazardous effects extensively being studied, rare attention has been paid to the potential protective effect of Ag-nps to human health. The present study was designed to evaluate the protective effects of Ag-nps and heat shock treatment on tumor necrosis factor-α (TNF-α)-induced cell damage in Clone 9 cells. Clone 9 cells were pretreated with nonlethal concentration of Ag-nps (1 μg/ml) or heat shock, and then cell damages were induced by TNF-α (1 ng/ml). Protective effects of Ag-nps administration or heat shock treatment were determined by examining the TNF-α-induced changes in cell viabilities. The results showed that the intensity of cytotoxicity produced by TNF-α was alleviated upon treatment with nonlethal concentration of Ag-nps (1 μg/ml). Similar protective effects were also found upon heat shock treatment. These data demonstrate that Ag-nps and heat shock treatment were equally capable of inducing heat shock protein 70 (HSP70) protein expression in Clone 9 cells. The results suggest that clinically Ag-nps administration is a viable strategy to induce endogenous HSP70 expression instead of applying heat shock. In conclusion, our study for the first time provides evidence that Ag-nps may act as a viable alternative for HSP70 induction clinically.

• Authors: Tsen-Ni Tsai, Tzu-Ying Lee, Maw-Shung Liu, Jia-Jing Ho, Li-Ju Huang, Chia-Jen Liu, Tsan-Ju Chen, Rei-Cheng Yang
• Journal: American journal of physiology. Cell physiology (Am J Physiol Cell Physiol) Vol. 308 Issue 12 Pg. C959-63 (Jun 15 2015) ISSN: 1522-1563 [Electronic] United States
• PMID: 25877698 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 the American Physiological Society.

Chemical References

• HSP70 Heat-Shock Proteins
• Tumor Necrosis Factor-alpha
• Silver

Topics

• Animals
• Cell Death (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Cytoprotection
• Dose-Response Relationship, Drug
• Epithelial Cells (drug effects, metabolism, pathology)
• HSP70 Heat-Shock Proteins (metabolism)
• Heat-Shock Response
• Hot Temperature
• Liver (drug effects, metabolism, pathology)
• Metal Nanoparticles
• Rats
• Silver (pharmacology)
• Time Factors
• Tumor Necrosis Factor-alpha (toxicity)
• Up-Regulation