Sodium salicylate reduced insulin resistance in the retina of a type 2 diabetic rat model.

Abstract

Sodium salicylate has been reported to reduce markers of diabetic retinopathy in a type 1 rat model. Because rates of type 2 diabetes are on the rise, we wanted to determine whether salicylate could improve insulin resistance in a type 2 rat model, as well as improve retinal function. We treated lean and obese BBZDR/Wor type 2 diabetic rats with salicylate in their chow for 2 months. Prior to salicylate treatment, rats underwent an electroretinogram to measure retinal function. After 2 months of treatment, rats underwent an additional electroretinogram prior to sacrifice. In addition to the animal model, we also treated retinal endothelial cells (REC) and rat Müller cells with salicylate and performed the same analyses as done for the rat retinal lysates. To investigate the role of salicylate in insulin signaling, we measured TNFα and caspase 3 levels by ELISA, as well as performed Western blotting for insulin receptor substrate 1, insulin receptor, SOCS3, and pro- and anti-apoptotic markers. Data demonstrated that salicylate significantly improved retinal function, as well as reduced TNFα and SOCS3-induced insulin resistance in all samples. Overall, results suggest that salicylate is effective in reducing insulin resistance in the retina of type 2 diabetic rat models.

• Authors: Youde Jiang, Shalini Thakran, Rajini Bheemreddy, William Coppess, Robert J Walker, Jena J Steinle
• Journal: PloS one (PLoS One) Vol. 10 Issue 4 Pg. e0125505 ( 2015) ISSN: 1932-6203 [Electronic] United States
• PMID: 25874611 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• I-kappa B Proteins
• Insulin
• Insulin Receptor Substrate Proteins
• NFKBIA protein, human
• Nfkbia protein, rat
• Socs3 protein, rat
• Suppressor of Cytokine Signaling 3 Protein
• Suppressor of Cytokine Signaling Proteins
• Tumor Necrosis Factor-alpha
• NF-KappaB Inhibitor alpha
• Receptor, Insulin
• Caspase 3
• Glucose
• Sodium Salicylate

Topics

• Animals
• Caspase 3 (analysis)
• Cells, Cultured
• Diabetes Mellitus, Type 2 (metabolism, pathology)
• Disease Models, Animal
• Electroretinography
• Endothelial Cells (cytology, drug effects, metabolism)
• Enzyme-Linked Immunosorbent Assay
• Ependymoglial Cells (cytology, drug effects, metabolism)
• Glucose (pharmacology)
• Humans
• I-kappa B Proteins (metabolism)
• Insulin (metabolism)
• Insulin Receptor Substrate Proteins (metabolism)
• Insulin Resistance
• Intraocular Pressure (physiology)
• Male
• NF-KappaB Inhibitor alpha
• Phosphorylation (drug effects)
• Rats
• Rats, Inbred BB
• Receptor, Insulin (metabolism)
• Retina (drug effects, metabolism)
• Signal Transduction (drug effects)
• Sodium Salicylate (pharmacology)
• Suppressor of Cytokine Signaling 3 Protein
• Suppressor of Cytokine Signaling Proteins (metabolism)
• Tumor Necrosis Factor-alpha (analysis)