Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke.

Abstract

PURPOSE:

Limb remote ischemic per-conditioning or post-conditioning has been shown to be neuroprotective after cerebral ischemic stroke. However, the effect of combining remote per-conditioning with post-conditioning on ischemic/reperfusion injury as well as the underlying mechanisms are largely unexplored.

METHODS:

Here, adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO). The limb ischemic stimulus was immediately applied after onset of focal ischemia (per-conditioning), followed by repeated short episodes of remote ischemia 24 hr after reperfusion (post-conditioning). The infarct volume, motor function, and the expression of neuroglobin (Ngb) were measured at different durations after reperfusion.

RESULTS:

We found that a single episode of limb remote per-conditioning afforded short-term protection, but combining repeated remote post-conditioning during the 14 days after reperfusion significantly ameliorated cerebral ischemia/reperfusion injury. Interestingly, we also found that ischemic per- and post-conditioning significantly increased expression of Ngb, an oxygen-binding globin protein that has been demonstrated to be neuroprotective against stroke, at peri-infarct regions from day 1 to day 14 following ischemia/reperfusion.

CONCLUSION:

Our results suggest that the conventional per-conditioning combined with post-conditioning may be used as a novel neuroprotective strategy against ischemia-reperfusion injury, and Ngb seems to be one of the important players in limb remote ischemia-mediated neuroprotection.

• Authors: Changhong Ren, Pengcheng Wang, Brian Wang, Ning Li, Weiguang Li, Chenggang Zhang, Kunlin Jin, Xunming Ji
• Journal: Restorative neurology and neuroscience (Restor Neurol Neurosci) Vol. 33 Issue 3 Pg. 369-79 ( 2015) ISSN: 1878-3627 [Electronic] Netherlands
• PMID: 25868435 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nerve Tissue Proteins
• Neuroglobin
• Globins

Topics

• Animals
• Brain (metabolism)
• Brain Ischemia (metabolism, therapy)
• Disease Models, Animal
• Globins (genetics, metabolism)
• Ischemic Postconditioning (methods)
• Ischemic Preconditioning (methods)
• Male
• Nerve Tissue Proteins (genetics, metabolism)
• Neuroglobin
• Rats
• Rats, Sprague-Dawley
• Stroke (metabolism, therapy)
• Treatment Outcome