Abstract |
Toxic shock syndrome toxin-1 (TSST-1) is one of superantigens produced by Staphylococcus aureus. We have previously demonstrated that vaccination with non-toxic mutant TSST-1 (mTSST-1) develops host protection to lethal S. aureus infection in mice. However, the detailed mechanism underlying this protection is necessary to elucidate because the passive transfer of antibodies against TSST-1 fails to provide complete protection against S. aureus infection. In this study, the results showed that interleukin-17A (IL-17A)-producing cells were increased in the spleen cells of mTSST-1-vaccinated mice. The main source of IL-17A in mTSST-1-vaccinated mice was T-helper 17 (Th17) cells. The protective effect of vaccination was induced when the vaccinated wild type but not IL-17A-deficient mice were challenged with S. aureus. Gene expression of chemokines, CCL2 and CXCL1, and infiltration of neutrophils and macrophages were increased in spleens and livers of vaccinated mice after infection. The IL-17A-dependent immune response was TSST-1 specific because TSST-1-deficient S. aureus failed to induce the response. The present study suggests that mTSST-1 vaccination is able to provide the IL-17A-dependent host defense against S. aureus infection which promotes chemokine-mediated infiltration of phagocytes into the infectious foci.
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Authors | Kouji Narita, Dong-Liang Hu, Krisana Asano, Akio Nakane |
Journal | Pathogens and disease
(Pathog Dis)
Vol. 73
Issue 4
(Jun 2015)
ISSN: 2049-632X [Electronic] United States |
PMID | 25857736
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © FEMS 2015. All rights reserved. For permissions, please e-mail: [email protected]. |
Chemical References |
- Bacterial Toxins
- Enterotoxins
- Il17a protein, mouse
- Interleukin-17
- Staphylococcal Vaccines
- Superantigens
- enterotoxin F, Staphylococcal
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Topics |
- Animals
- Bacterial Toxins
(immunology)
- Enterotoxins
(immunology)
- Interleukin-17
(metabolism)
- Mice
- Spleen
(immunology)
- Staphylococcal Infections
(prevention & control)
- Staphylococcal Vaccines
(administration & dosage, immunology)
- Staphylococcus aureus
(immunology)
- Superantigens
(immunology)
- Th17 Cells
(immunology)
- Vaccination
(methods)
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