Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice.
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| Abstract |
Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A.
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| Authors | Naoya Yamashita, Aoi Jitsuki-Takahashi, Miyuki Ogawara, Wataru Ohkubo, Tomomi Araki, Chie Hotta, Tomohiko Tamura, Shu-ichi Hashimoto, Takashi Yabuki, Toru Tsuji, Yukie Sasakura, Hiromi Okumura, Aki Takaiwa, Chika Koyama, Koji Murakami, Yoshio Goshima |
| Journal | International immunology (Int Immunol) Vol. 27 Issue 9 Pg. 459-66 (Sep 2015) ISSN: 1460-2377 [Electronic] England |
| PMID | 25855660 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © The Japanese Society for Immunology. 2015. All rights reserved. For permissions, please e-mail: [email protected]. |
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