Abstract | OBJECTIVE: METHODS: RESULTS: The healing rate of corneal epithelium in EGCG group was significantly higher than that of PBS group at d1, d3 and d7 after treatment (d1: 41.0%±13.0% vs 23.8%±7.6%; d3: 76.6%±7.5% vs 61.2%±6.8%; d7: 87.8%±8.5% vs 74.0%±9.1%; all P <0.05). The CNV scores and the number of CNV in the corneal sections of EGCG group were significantly lower than those of PBS group at d3, d7 and d14 after treatment (CNV score: d3: 1.1±0.5 vs 6.6±1.0; d7: 1.3±0. 3 vs 8.1±1.0; d14: 0.9±0.2 vs 9.2±1.1; CNV number: d3: 1.68±0.61 vs 2.92±0.95; d7: 4.80±1.36 vs 7.92±1.28; d14: 3.64±0.71 vs 5.88±0.76; all P<0.05) . The expression of VEGF protein at d3 (0.19±0.05 vs 0.45±0.08) and d7 (0.42±0.07 vs 0.84±0.09), the expression of VEGF mRNA at d1, d3 and d7 in EGCG group were significantly lower than those in PBS group (all P <0.05). Compared to PBS group, the inflammatory index at d3 (3.2±0.4 vs 3.7±0.5) and d7 (2.3±0.5 vs 4.0±0.0), the number of PMNs in the corneal sections and the MPO values at d3, d7 and d14 in EGCG group were significantly decreased (PMNs: d3: 34.5±15.7 vs 90.0±28.8; d7: 17.1±11.4 vs 54.9±25.9; d14: 12. 8±4.6 vs 39.0±17.9; all P <0.05). CONCLUSION: In the murine corneal alkali burn model, intraperitoneal injection of EGCG solution can promote the healing of corneal epithelium, inhibit the formation of CNV and reduce the inflammatory cell infiltration in the corneas.
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Authors | Lian-Qun Wu, Min Lu |
Journal | Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
(Zhejiang Da Xue Xue Bao Yi Xue Ban)
Vol. 44
Issue 1
Pg. 15-23
(01 2015)
ISSN: 1008-9292 [Print] China |
PMID | 25851970
(Publication Type: Journal Article)
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Chemical References |
- Alkalies
- RNA, Messenger
- Vascular Endothelial Growth Factor A
- vascular endothelial growth factor A, mouse
- Catechin
- epigallocatechin gallate
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Topics |
- Alkalies
- Animals
- Burns, Chemical
(drug therapy)
- Catechin
(analogs & derivatives, therapeutic use)
- Cornea
(drug effects, pathology)
- Corneal Neovascularization
(prevention & control)
- Disease Models, Animal
- Eye Burns
(drug therapy)
- Inflammation
(drug therapy, immunology)
- Mice
- Mice, Inbred C57BL
- Neutrophils
(cytology)
- RNA, Messenger
- Vascular Endothelial Growth Factor A
(metabolism)
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