The purpose of this study was to compare
messenger RNA (
mRNA) expression of
selenoprotein genes between
hepatoma HepG2 and normal hepatocytes LO2 cell lines. Liver HepG2 and LO2 cells were cultured in 12-well plates under the same condition until cells grew to complete confluence, and then cells were harvested for total
RNA and
protein extraction. The qPCRs were performed to compare gene expression of 14
selenoprotein genes and 5
cancer signaling-related genes.
Enzyme activities were also assayed. The results showed that human
hepatoma HepG2 cells grew faster than normal hepatocytes LO2 cells. Among the genes investigated, 10
selenoprotein genes (Gpx1, Gpx3, Gpx4, Selx, Sepp, Sepw1, Sepn1, Selt, Seli, Selh) and 3
cancer signaling-related genes (Bcl-2A,
caspase-3, and P38) were upregulated (P < 0.05), while Selo and Bcl-2B were downregulated (P < 0.05) in
hepatoma HepG2 cells compared to LO2 cells. Significant correlations were found between
selenoprotein genes and the
cancer signaling-related genes Caspase3, P53, Bc1-2A, and Bc1-2B. Our results revealed that
selenoprotein genes were aberrantly expressed in
hepatoma HepG2 cells compared to normal liver LO2 cells, which indicated that those
selenoprotein genes may play important roles in the occurrence and development of liver
carcinogenesis.