Abstract | PURPOSE: METHODS:
Dexamethasone nanomicelles were prepared and characterized for critical micellar concentration, solubility of dexamethasone, particle size, surface charge, morphology, in vitro drug release, clarity, stability, filtration efficiency, and sterility. Ocular tolerance and the tissue drug distribution of dexamethasone were assessed in rabbits after single and multiple topical administration. RESULTS:
Dexamethasone nanomicelles (0.1% w/v) were successfully developed and characterized with an optimized composition of P40S/P80=7/3 by weight. The mean diameter of blank and drug-loaded nanomicelles was 13.3±0.4 and 14.5±0.4 nm, respectively. Transmission electron microscopy images revealed the spherical structure of nanomicelles. Nanomicelles were found to be stable with respect to clarity, size and drug content at 4°C and 25°C for up to 6 months. No irritation or redness was observed in the treated eyes as compared with the untreated control rabbit eyes. Therapeutic concentrations of dexamethasone were observed in the retina and choroid after single and multiple topical application in rabbits. CONCLUSION: In conclusion, the nanomicelles of P40S and P80 could efficiently solubilize 0.1% dexamethasone in their cores. The results also indicate that mixed nanomicelles could be utilized as a potential delivery system for delivering dexamethasone to treat the back of the eye diseases such as posterior uveitis after topical application.
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Authors | Soohi Patel, Chandrasekhar Garapati, Pallabita Chowdhury, Himanshu Gupta, Jerry Nesamony, Surya Nauli, Sai H S Boddu |
Journal | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
(J Ocul Pharmacol Ther)
Vol. 31
Issue 4
Pg. 215-27
(May 2015)
ISSN: 1557-7732 [Electronic] United States |
PMID | 25839185
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glucocorticoids
- Micelles
- Dexamethasone
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Topics |
- Administration, Ophthalmic
- Animals
- Calorimetry, Differential Scanning
(methods)
- Dexamethasone
(administration & dosage, chemistry, pharmacokinetics)
- Drug Delivery Systems
- Glucocorticoids
(administration & dosage, chemistry, pharmacokinetics)
- Male
- Micelles
- Microscopy, Electron, Transmission
(methods)
- Nanoparticles
(administration & dosage, chemistry)
- Rabbits
- Solubility
- Uveitis, Posterior
(drug therapy, metabolism, pathology)
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