Abstract | BACKGROUND: OBJECTIVES: The aim of the present study was to evaluate the antigen-detection capacity in the stool specimens using ELISA and oocyst detection by AP staining methods, for the diagnosis of human cryptosporidiosis. MATERIALS AND METHODS: A total of 228 fecal samples were collected from residents of rural areas of Hamadan, West of Iran. Each fecal sample was divided into two parts, one kept frozen at -20˚C for Ag-capture ELISA and the other in 10% formalin for the AP staining method. Cryptosporidium Ag-detection ELISA procedure was performed according to the manual of the manufacturer. The preserved samples concentrated using the formalin- ether concentration technique were stained with AP and then investigated under florescent microscopy. RESULTS: Eight (3.5%) and three (1.3%) out of 228 fecal samples were positive for Cryptosporidium infection by ELISA and AP staining methods, respectively. Cryptosporidium Ag-detection using ELISA showed an increased frequency of the infection, compared to the AP staining method (P = 0.062). CONCLUSIONS: For epidemiological studies and diagnostic purposes of the Cryptosporidium infection, especially in asymptomatic individuals, Ag-detection ELISA is an easy to perform and accurate method, compared to other conventional microscopic methods.
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Authors | Rasool Jafari, Amir Hossein Maghsood, Marzieh Safari, Milad Latifi, Mohammad Fallah |
Journal | Jundishapur journal of microbiology
(Jundishapur J Microbiol)
Vol. 8
Issue 2
Pg. e16470
(Feb 2015)
ISSN: 2008-3645 [Print] Netherlands |
PMID | 25825642
(Publication Type: Journal Article)
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