Loss of expression of cyclin d2 by aberrant DNA methylation: a potential biomarker in vietnamese breast cancer patients.

Abstract

DNA methylation of tumor suppressor gene promoters is the most frequent phenomenon leading to inactivation of function, consequently driving malignant cell transformation. Cyclin D2 is implicated in tumor suppression. In our study, we carried out the MSP assay to evaluation the methylation status at CpG islands in the cyclin D2 promoter in breast cancer cases from the Vietnamese population. The results showed that the frequency of methylation reached 62.1% (59 of 95 breast cancer tumors), but was low in non-cancer specimens at 10% (2 of 20 non-cancer specimens). Additionally, with an RR (relative risk) and OR (odd ratios) of 6.21 and 14.8, DNA hypermethylation of cyclin D2 increased the possibility of malignant transformation. Our results confirmed the cyclin D2 hypermethylation could be used as the potential biomarker which could be applied in prognosis and early diagnosis of Vietnamese breast cancer patients.

• Authors: Phuong Kim Truong, Thuan Duc Lao, Thao Phuong Thi Doan, Thuy Ai Huyen Le
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 16 Issue 6 Pg. 2209-13 ( 2015) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25824739 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• CCND2 protein, human
• Cyclin D2

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (genetics)
• Breast Neoplasms (diagnosis, epidemiology, genetics)
• Case-Control Studies
• CpG Islands (genetics)
• Cyclin D2 (genetics)
• DNA Methylation
• Female
• Follow-Up Studies
• Humans
• Middle Aged
• Neoplasm Grading
• Neoplasm Staging
• Prognosis
• Promoter Regions, Genetic (genetics)
• Vietnam (epidemiology)