Induction of Sd(a)-sialomucin and sulfated H-sulfomucin in mouse small intestinal mucosa by infection with parasitic helminth.

Abstract

Mucin is a major component of mucus on gastrointestinal mucosa. Mucin alteration in the host is considered to be the principal event for expulsion of intestinal helminths. However, it is unclear what mucin alterations are induced by various helminth infections. In this study, the alterations of mouse small intestinal mucin after infection with two nematodes, Nippostrongylus brasiliensis and Heligmosomoides polygyrus, which parasitize the jejunal epithelium, and a cestode, Vampirolepis nana, which parasitizes the ileal epithelium, were examined biochemically and histologically using two anti-mucin monoclonal antibodies (mAbs), HCM31 and PGM34, which recognize Sd(a) antigen, NeuAcα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ-, and sulphated H type 2 antigen, Fucα1-2Galβ1-4GlcNAc(6SO₃H)β-, respectively. The goblet cell mucins that reacted with HCM31 increased conspicuously on the jejunal mucosa concurrently with expulsion of N. brasiliensis. Increased levels of HCM31-reactive mucins were observed in the jejunal mucosa after H. polygyrus infection, despite the ongoing parasitism. Goblet cell mucins that reacted with PGM34 increased on the ileal mucosa during V. nana parasitism. Small intestinal goblet cells reacting with the two mAbs were not observed in non-infected mice, although sialomucins and sulfomucins were abundantly present. Additionally, the number of ileal goblet cells that reacted with the two mAbs was increased at the time of expulsion of heterophyid trematode. These results indicate that the type of specific acidic mucins expressed after infection varies among species of intestinal helminth, and, furthermore, that the relationship with worm expulsion is also different.

• Authors: Daigo Tsubokawa, Kenji Ishiwata, Yukinobu Goso, Takuya Yokoyama, Hirotaka Kanuka, Kazuhiko Ishihara, Takeshi Nakamura, Naotoshi Tsuji
• Journal: Experimental parasitology (Exp Parasitol) Vol. 153 Pg. 165-73 (Jun 2015) ISSN: 1090-2449 [Electronic] United States
• PMID: 25819298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Mucins
• Sialomucins
• sulfomucin

Topics

• Animals
• Goblet Cells (metabolism, parasitology, pathology)
• Humans
• Intestinal Mucosa (metabolism, parasitology, pathology)
• Jejunum (metabolism, parasitology, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Mucins (genetics, metabolism)
• Nematospiroides dubius (physiology)
• Nippostrongylus (physiology)
• Sialomucins (genetics, metabolism)
• Strongylida Infections (genetics, metabolism, parasitology, pathology)