A bioactive film based on cashew gum polysaccharide for wound dressing applications.

Abstract

This work presents the development of a new bioactive material for wound therapeutics which may play a dual role of modulate metallo proteinases activity while prevents infection blocking out pathogenic microorganisms and foreign materials. A CGP/PVA film was activated by covalent immobilization of trypsin. Results from biocompatibility test revealed that PDL fibroblasts grown on the surface of CGP/PVA and the high amount of viable cells proved absence of cytotoxicity. Trypsin immobilized onto CGP/PVA film remained 100% active after 28 days stored dried at room temperature. In addition, CGP/PVA-trypsin film could be used for 9 cycles of storage/use without loss of activity. After immobilization, trypsin retained its collagenolytic activity, indicating this material as a promising material for wound dressing applications.

• Authors: Bruna R Moreira, Karla A Batista, Elisandra G Castro, Eliana M Lima, Kátia F Fernandes
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 122 Pg. 69-76 (May 20 2015) ISSN: 1879-1344 [Electronic] England
• PMID: 25817644 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Biocompatible Materials
• Enzymes, Immobilized
• Plant Gums
• Polysaccharides
• Polyvinyl Alcohol
• Collagen
• Trypsin

Topics

• Anacardium (chemistry)
• Animals
• Bandages (adverse effects)
• Biocompatible Materials (adverse effects, chemistry, isolation & purification, pharmacology)
• Cattle
• Cell Survival (drug effects)
• Collagen (metabolism)
• Enzyme Stability
• Enzymes, Immobilized (chemistry, metabolism)
• Humans
• Hydrogen-Ion Concentration
• Hydrolysis
• Models, Molecular
• Oxidation-Reduction
• Plant Gums (chemistry)
• Polysaccharides (adverse effects, chemistry, isolation & purification, pharmacology)
• Polyvinyl Alcohol (chemistry)
• Protein Conformation
• Temperature
• Trypsin (chemistry, metabolism)
• Wound Healing (drug effects)