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Characterization of GM-CSF-inhibitory factor and Uracil DNA glycosylase encoding genes from camel pseudocowpoxvirus.

Abstract
The present study describes the PCR amplification of GM-CSF-inhibitory factor (GIF) and Uracil DNA glycosylase (UDG) encoding genes of pseudocowpoxvirus (PCPV) from the Indian Dromedaries (Camelus dromedarius) infected with contagious ecthyma using the primers based on the corresponding gene sequences of human PCPV and reindeer PCPV, respectively. The length of GIF gene of PCPV obtained from camel is 795 bp and due to the addition of one cytosine residue at position 374 and one adenine residue at position 516, the open reading frame (ORF) got altered, resulting in the production of truncated polypeptide. The ORF of UDG encoding gene of camel PCPV is 696 bp encoding a polypeptide of 26.0 kDa. Comparison of amino acid sequence homologies of GIF and UDG of camel PCPV revealed that the camel PCPV is closer to ORFV and PCPV (reference stains of both human and reindeer), respectively.
AuthorsG Nagarajan, Shelesh Kumar Swami, Shyam Singh Dahiya, S D Narnaware, S C Mehta, P K Singh, Raghvendar Singh, F C Tuteja, N V Patil
JournalResearch in veterinary science (Res Vet Sci) Vol. 100 Pg. 291-6 (Jun 2015) ISSN: 1532-2661 [Electronic] England
PMID25816930 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier Ltd. All rights reserved.
Chemical References
  • Viral Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Uracil-DNA Glycosidase
Topics
  • Amino Acid Sequence
  • Animals
  • Camelus
  • Ecthyma, Contagious (virology)
  • Female
  • Granulocyte-Macrophage Colony-Stimulating Factor (antagonists & inhibitors, genetics, metabolism)
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction (veterinary)
  • Poxviridae Infections (veterinary, virology)
  • Pseudocowpox Virus (genetics, metabolism)
  • Sequence Alignment (veterinary)
  • Sequence Homology, Amino Acid
  • Uracil-DNA Glycosidase (genetics, metabolism)
  • Viral Proteins (genetics, metabolism)

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