Functional and dynamic state of inner mitochondrial membrane of sarcoma 37 in mice under administration of sodium dichloroacetate.

Abstract

The activity of enzymes of the respiratory chain and structural-dynamic properties of the inner mitochondrial membrane (IMM) of sarcoma 37 (S37) in mice under sodium dichloroacetate (SDA) administration in a daily dose of 86 mg/kg of body weight starting from the 2nd day after tumor transplantation were investigated. The dynamic and structural state of the IMM components was determined using the fluorescent probes. With S37 growth the intensification of glycolytic metabolism occurred on the background of suppressed functional capacity of mitochondrial respiratory chain enzymes. The changes of conformational properties of protein molecules and the increase of IMM lipid phase microviscosity were shown. The administration of SDA promotes the decrease of lactate content and the increase of pyruvate dehydrogenase activity in S37. This was accompanied by further suppression of the functional activity of the respiratory chain complexes and H+-ATPase coupled with conformational modification ofprotein molecules and changes of the structural orderliness of the IMM lipid phase, possibly due to intensification of reactive oxygen species generation.

• Authors: S V Khyzhnyak, L V Sorokina, L I Stepanova, A A Kaplia
• Journal: Ukrainian biochemical journal (Ukr Biochem J) 2014 Nov-Dec Vol. 86 Issue 6 Pg. 106-18 ISSN: 2409-4943 [Print] Ukraine
• PMID: 25816611 (Publication Type: Journal Article)

Chemical References

• Antimetabolites, Antineoplastic
• Reactive Oxygen Species
• Lactic Acid
• Dichloroacetic Acid
• Ketone Oxidoreductases
• pyruvate dehydrogenase (NADP+)
• Protein Serine-Threonine Kinases
• Proton-Translocating ATPases

Topics

• Animals
• Antimetabolites, Antineoplastic (pharmacology)
• Dichloroacetic Acid (pharmacology)
• Electron Transport (drug effects)
• Glycolysis (drug effects)
• Ketone Oxidoreductases (metabolism)
• Lactic Acid (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Mitochondrial Membranes (chemistry, drug effects, metabolism)
• Muscle Neoplasms (drug therapy, metabolism, pathology)
• Neoplasm Transplantation
• Protein Conformation
• Protein Serine-Threonine Kinases (metabolism)
• Proton-Translocating ATPases (metabolism)
• Reactive Oxygen Species (agonists, metabolism)
• Sarcoma (drug therapy, metabolism, pathology)
• Tumor Burden (drug effects)