Controlling the origins of inflammation with a photoactive lipopeptide immunopotentiator.

Abstract

Inflammatory immune responses are mediated by signaling molecules that are both produced by and recognized across highly heterogeneous cell populations. As such, the study of inflammation using traditional immunostimulants is complicated by paracrine and autocrine signaling, which obscures the origin of a propagating response. To address this challenge, we developed a small-molecule probe that can photosensitize immune cells, thus allowing light-mediated inflammation. This probe was used to control the origin of inflammation using light. Following this motif, inflammation was initiated from fibroblasts or dendritic cells. The contributions of fibroblasts and dendritic cells in initiating inflammation in heterogeneous co-culture are reported, thus providing insights into the future development of vaccines and treatment of inflammation.

• Authors: Rock J Mancini, Lalisa Stutts, Troy Moore, Aaron P Esser-Kahn
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 54 Issue 20 Pg. 5962-5 (May 11 2015) ISSN: 1521-3773 [Electronic] Germany
• PMID: 25800006 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Lipopeptides
• Molecular Probes

Topics

• Coculture Techniques
• Dendritic Cells (immunology)
• Fibroblasts (immunology)
• Humans
• Inflammation (immunology)
• Lipopeptides (chemical synthesis, chemistry, immunology)
• Models, Molecular
• Molecular Probes (chemical synthesis, chemistry, immunology)
• Molecular Structure
• Photochemical Processes