Laboratory confirmation of clinically diagnosed malaria in a cohort of HIV-infected mothers and their children in Malawi.

Abstract

To avoid overdiagnosis, accuracy in the identification of true malaria cases is of critical importance. Samples (either whole blood, dried blood spots or plasma/serum) collected at the time of clinically diagnosed malaria episodes in a cohort of Malawian HIV-infected mothers and their children were retrospectively tested with the enzyme-linked immunosorbent assay (ELISA) for HRP-2 (histidine-rich protein 2) detection. There were 55 and 56 clinically diagnosed cases of malaria in mothers and children, respectively, with samples available for testing. Rates of laboratory-confirmed episodes were 20% (11 of 55) in mothers and 16.1% (9 of 56) in children. Hemoglobin was lower in children with confirmed malaria compared to those with clinical malaria diagnosis. The results of our study support the widespread use of rapid diagnostic tests.

• Authors: Marina Giuliano, Clementina Maria Galluzzo, Sandro Mancinelli, Mauro Andreotti, Haswel Jere, Jean-Baptiste Sagno, Martin Maulidi, Fulvio Erba, Roberta Amici, Ersilia Buonomo, Paola Scarcella, Maria Cristina Marazzi, Stefano Vella, Leonardo Palombi
• Journal: Journal of tropical pediatrics (J Trop Pediatr) Vol. 61 Issue 3 Pg. 222-5 (Jun 2015) ISSN: 1465-3664 [Electronic] England
• PMID: 25797059 (Publication Type: Letter, Research Support, Non-U.S. Gov't)
• Copyright: © The Author [2015]. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected].

Chemical References

• Antigens, Protozoan
• Proteins
• histidine-rich proteins

Topics

• Adult
• Animals
• Antigens, Protozoan (blood)
• Child
• Child, Preschool
• Enzyme-Linked Immunosorbent Assay
• Female
• HIV Infections (complications, diagnosis)
• HIV-1
• Humans
• Malaria (blood, diagnosis)
• Male
• Mothers
• Plasmodium (immunology, isolation & purification)
• Pregnancy
• Pregnancy Complications
• Proteins (analysis)
• Retrospective Studies