Levels of angiogenesis-related vascular endothelial growth factor family in neovascular glaucoma eyes.

Abstract

PURPOSE:

This study aimed to evaluate the angiogenesis-related factors of the vascular endothelial growth factor (VEGF) family in the aqueous humour of patients with neovascular glaucoma (NVG).

METHODS:

This study involved 22 eyes of 22 patients with advanced NVG requiring antiglaucomatous surgery and 20 control subjects with cataracts. The NVG eyes received an intravitreal injection of ranibizumab (IVR) treatment before antiglaucomatous surgery. Aqueous humour and blood were collected at the time of IVR and cataract surgery. Protein concentration of VEGF-A, VEGF-B and placenta growth factor (PlGF) in aqueous humour and plasma was determined by ELISA tests.

RESULTS:

The mean concentration (standard deviation) of VEGF-A and PlGF in the aqueous humour of patients with NVG were 3037 (2387) pg/ml and 1078 (712) pg/ml, respectively; both were significantly higher than the control group (both p < 0.001). However, levels of VEGF-A and PlGF in the serum of NVG and control subjects remained low. High concentrations of VEGF-A were closely correlated with high levels of PlGF in patients with NVG (r = 0.593, p = 0.004). Concentrations of VEGF-B in aqueous humour and serum remained unchanged (p > 0.05).

CONCLUSION:

There were high concentrations of angiogenesis factors of the VEGF family, with the exception of VEGF-B, in the aqueous humour of patients with NVG, and there was a positive correlation between VEGF-A and PlGF. High PlGF levels in patients with NVG may provide another potential target for treatment of NVG.

• Authors: Shida Chen, Minwen Zhou, Wei Wang, Huimin Wu, Xiling Yu, Wenbin Huang, Xinbo Gao, Jiawei Wang, Xingyi Li, Shaolin Du, Xiaoyan Ding, Xiulan Zhang
• Journal: Acta ophthalmologica (Acta Ophthalmol) Vol. 93 Issue 7 Pg. e556-60 (Nov 2015) ISSN: 1755-3768 [Electronic] England
• PMID: 25783445 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

Chemical References

• Angiogenesis Inhibitors
• PGF protein, human
• Pregnancy Proteins
• VEGFA protein, human
• VEGFB protein, human
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factor B
• Placenta Growth Factor
• Ranibizumab

Topics

• Adult
• Aged
• Angiogenesis Inhibitors (therapeutic use)
• Aqueous Humor (metabolism)
• Cataract (blood)
• Enzyme-Linked Immunosorbent Assay
• Female
• Glaucoma, Neovascular (blood, drug therapy)
• Humans
• Intravitreal Injections
• Male
• Middle Aged
• Placenta Growth Factor
• Pregnancy Proteins (blood)
• Prospective Studies
• Ranibizumab (therapeutic use)
• Vascular Endothelial Growth Factor A (blood)
• Vascular Endothelial Growth Factor B (blood)