Abstract |
Recent findings have suggested that microRNAs may be involved in the regulation of metastasis in malignant cancers such as non-small cell lung cancer (NSCLC). This study aimed to determine the relationship between expression of miR-125b and matrix metalloproteinase 13 (encoded by MMP-13) and the metastatic potential of cancer cells in NSCLC. Expression levels of miR-125b transcripts and MMP-13 proteins were analyzed by quantitative real-time PCR and immunohistochemistry, respectively, in tumor tissues and adjacent nontumor tissues from 42 patients with NSCLC. The interaction between miR-125b and MMP-13 expression and the associations between miR-125b and clinicopathologic data were analyzed. MiR-125 b expression levels were decreased in NSCLC tumor tissue samples, which correlated with an increased incidence of lymph node metastases, increased pathologic stage, increased MMP-13 expression levels, and decreased early progression-free survival. Additionally, we have demonstrated that increased levels of miR-125b can directly downregulate MMP-13 protein expression and inhibit the invasive capabilities of cancer cells. Expression levels of miR-125b were negatively correlated with metastatic potential of NSCLC tumors, which may function through regulation of MMP-13.
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Authors | Xiaozhou Yu, Feng Wei, Jinpu Yu, Hua Zhao, Lei Jia, Yingnan Ye, Ruijuan Du, Xiubao Ren, Hui Li |
Journal | Cancer genetics
(Cancer Genet)
Vol. 208
Issue 3
Pg. 76-84
(Mar 2015)
ISSN: 2210-7762 [Print] United States |
PMID | 25772251
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- MIRN125 microRNA, human
- MicroRNAs
- MMP13 protein, human
- Matrix Metalloproteinase 13
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Topics |
- Adult
- Carcinoma, Non-Small-Cell Lung
(secondary)
- Cell Line, Tumor
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Lung Neoplasms
(pathology)
- Lymphatic Metastasis
- Male
- Matrix Metalloproteinase 13
(physiology)
- MicroRNAs
(physiology)
- Middle Aged
- Neoplasm Staging
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