The potential protective role of caveolin-1 in intestinal inflammation in TNBS-induced murine colitis.

Abstract

BACKGROUND:

Caveolin-1 (Cav-1) is a multifunctional scaffolding protein serving as a platform for the cell's signal-transduction and playing an important role in inflammation. However, its role in inflammatory bowel disease is not clear. A recent study showed that Cav-1 is increased and mediates angiogenesis in dextran sodium sulphate-induced colitis, which are contradictory to our pilot findings in 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. In the present study, we further clarified the role of Cav-1 in TNBS-induced colitis.

METHODS:

In BALB/c mice, acute colitis was induced by intra-rectal administration of one dose TNBS, while chronic colitis was induced by administration of TNBS once a week for 7 weeks. To assess the effects of complete loss of Cav-1, Cav-1 knockout (Cav-1-/-) and control wild-type C57 mice received one TNBS administration. Body weight and clinical scores were monitored. Colon Cav-1 and pro-inflammatory cytokine levels were quantified through ELISAs. Inflammation was evaluated through histological analysis.

RESULTS:

Colon Cav-1 levels were significantly decreased in TNBS-induced colitis mice when compared to normal mice and also inversely correlated with colon inflammation scores and proinflammatory cytokine levels (IL-17, IFN-γ and TNF) significantly. Furthermore, after administration of TNBS, Cav-1-/- mice showed significantly increased clinical and colon inflammatory scores and body weight loss when compared with control mice.

CONCLUSIONS AND SIGNIFICANCE:

Cav-1 may play a protective role in the development of TNBS-induced colitis. Our findings raise an important issue in the evaluation of specific molecules in animal models that different models may exhibit opposite results because of the different mechanisms involved.

• Authors: Carolyn R Weiss, Qingdong Guan, Yanbing Ma, Gefei Qing, Charles N Bernstein, Richard J Warrington, Zhikang Peng
• Journal: PloS one (PLoS One) Vol. 10 Issue 3 Pg. e0119004 ( 2015) ISSN: 1932-6203 [Electronic] United States
• PMID: 25756273 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Caveolin 1
• Cytokines
• Trinitrobenzenesulfonic Acid
• Collagen

Topics

• Animals
• Caveolin 1 (physiology)
• Colitis (chemically induced, immunology, metabolism)
• Collagen (metabolism)
• Cytokines (metabolism)
• Female
• Male
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Mice, Knockout
• Trinitrobenzenesulfonic Acid