Abstract | BACKGROUND: MATERIALS AND METHODS: MRTF-A, AQP1 and neointima expression was detected both in the wire injured femoral arteries of wild-type mice and the atherosclerotic aortic tissues of ApoE-/- mice. Expression of ICAM-1, matrix metallopeptidase 9 (MMP-9) and integrin β1 were also assayed. The intercourse relationship between the molecules were investigated by interfering RNA and inhibitor assay. RESULTS: MRTF-A and AQP1 expression were significantly higher in the wire injured femoral arteries of wild-type mice and in the atherosclerotic aortic tissues of ApoE-/- mice than in healthy control tissues. Both in wire-injured femoral arteries in MRTF-A knockout (Mkl1-/-) mice and atherosclerotic lesions in Mkl1-/-; ApoE-/- mice, neointima formation were significantly attenuated and the expression of AQP1 were significantly decreased. Expression of ICAM-1, matrix metallopeptidase 9 (MMP-9) and integrin β1, three SRF targets and key regulators of cell migration, and AQP1 in injured arteries was significantly weaker in Mkl1-/- mice than in wild-type mice. In cultured vascular smooth muscle cells (VSMCs), knocking down MRTF-A reduced expression of these genes and significantly impaired cell migration. Underlying the increased MRTF-A expression in dedifferentiated VSMCs were the down-regulation of microRNA-300. Moreover, the MRTF-A inhibitor CCG1423 significantly reduced neointima formation following wire injury in mice. CONCLUSIONS:
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Authors | Yong Jiang |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 16
Issue 4
Pg. 1375-83
( 2015)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 25743802
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)
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Chemical References |
- Apolipoproteins E
- Aqp1 protein, mouse
- Mrtfa protein, mouse
- RNA, Messenger
- Trans-Activators
- Aquaporin 1
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Topics |
- Animals
- Apolipoproteins E
(physiology)
- Apoptosis
- Aquaporin 1
(genetics, metabolism)
- Atherosclerosis
(etiology, pathology)
- Blotting, Western
- Cell Adhesion
- Cell Movement
- Cell Proliferation
- Cells, Cultured
- Female
- Femoral Artery
(injuries, pathology)
- Immunoprecipitation
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Muscle, Smooth, Vascular
(injuries, pathology)
- Neointima
(etiology, pathology)
- RNA, Messenger
(genetics)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
- Trans-Activators
(physiology)
- Vascular Remodeling
- Wound Healing
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