Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.

Abstract

Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.

• Authors: Ming Yuan, Wensheng Zhang, Jun Wang, Chadwan Al Yaghchi, Jahangir Ahmed, Louisa Chard, Nick R Lemoine, Yaohe Wang
• Journal: Journal of virology (J Virol) Vol. 89 Issue 9 Pg. 5176-9 (May 2015) ISSN: 1098-5514 [Electronic] United States
• PMID: 25741005 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Topics

• CRISPR-Cas Systems
• Genome, Viral
• Molecular Biology (methods)
• Recombination, Genetic
• Technology, Pharmaceutical (methods)
• Vaccinia virus (genetics)