Abstract |
The use of adenovirus vector-based vaccines is a promising approach for generating antigen-specific immune responses. Improving vaccine potency is necessary in other approaches to address their inadequate protection for the majority of infectious diseases. This study is the first to reconstruct a recombinant replication-defective human adenovirus co-expressing E2 and invasin C-terminal (InvC) glycoproteins (rAd-E2-InvC). rAd-E2-InvC with 2 x 10(6) TCID50 was intramuscularly administered two times to CSFV-free pigs at 14 day intervals. No adverse clinical reactions were observed in any of the pigs after the vaccination. The CSFV E2-specific antibody titer was significantly higher in the rAd-E2-InvC group than that in the rAdV-E2 group as measured by NPLA and blocking ELISA. Pigs immunized with rAd-E2-InvC were completely protected against lethal challenge. Neither CSFV RNA nor pathological changes were detected in the tissues after CSFV challenge. These results demonstrate that rAd-E2-InvC could be an alternative to the existing CSF vaccine. Moreover, InvC that acts as an adjuvant could enhance the immunogenicity of rAdV-E2 and induce high CSFV E2-specific antibody titer and protection level.
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Authors | Helin Li, Pengbo Ning, Zhi Lin, Wulong Liang, Kai Kang, Lei He, Yanming Zhang |
Journal | Journal of biosciences
(J Biosci)
Vol. 40
Issue 1
Pg. 79-90
(Mar 2015)
ISSN: 0973-7138 [Electronic] India |
PMID | 25740144
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adenovirus E2 Proteins
- Adhesins, Bacterial
- Antibodies, Viral
- Glycoproteins
- Viral Envelope Proteins
- Viral Vaccines
- invasin, Yersinia
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Topics |
- Adenovirus E2 Proteins
(immunology)
- Adenoviruses, Human
(immunology)
- Adhesins, Bacterial
(biosynthesis, genetics, immunology)
- Animals
- Antibodies, Viral
(immunology)
- Cell Line
- Classical Swine Fever
(immunology)
- Classical Swine Fever Virus
(immunology)
- Glycoproteins
(biosynthesis, immunology)
- HEK293 Cells
- Humans
- Random Allocation
- Swine
- Vaccination
- Vaccine Potency
- Viral Envelope Proteins
(genetics, immunology)
- Viral Vaccines
(immunology)
- Virus Replication
(genetics)
- Yersinia enterocolitica
(immunology)
- Yersinia pseudotuberculosis
(immunology)
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