Abstract | BACKGROUND: MATERIALS AND METHODS: The human thyroid carcinoma cell line ARO, FRO, WRO, and KAT-5, were infected with G47Δat different multiplicities of infection (MOIs) in vitro. The survival rates of infected cells were calculated each day. Two s.c. tumor models were established using ARO and FRO cells in Balb/c nude mice, which were intratumorally (i.t.) treated with either G47Δor mock. Tumor volumes and mouse survival times were documented. RESULTS: G47Δ was highly cytotoxic to different types of thyroid carcinomas. For ARO, FRO, and KAT-5, greater than 30% and 80% of cells were killed at MOI=0.01 and MOI=0.1, respectively on day 5. WRO cells displayed modest sensitivity to G47Δ, with only 21% and 38% of cells killed. In the s.c. tumor model, both of the anaplastic thyroid carcinoma cell lines (ARO and FRO) were highly sensitive to G47Δ G47Δ significantly inhibited tumor growth and prolonged the survival of mice bearing s.c. ARO and FRO tumors. CONCLUSIONS:
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Authors | Jia-Ni Wang, Li-Hua Xu, Wei-Gen Zeng, Pan Hu, Samuel D Rabkin, Ren-Rin Liu |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 16
Issue 3
Pg. 1241-5
( 2015)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 25735362
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cell Proliferation
- Female
- Genetic Vectors
(administration & dosage)
- Humans
- Immunoenzyme Techniques
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Oncolytic Virotherapy
- Simplexvirus
(genetics)
- Thyroid Neoplasms
(genetics, pathology, therapy, virology)
- Tumor Burden
- Tumor Cells, Cultured
- Virus Replication
- Xenograft Model Antitumor Assays
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