Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp.

Abstract

Identification and characterization of the RNAi-related genes is the key to understanding RNAi mechanism in shrimp. In this study, we have identified and characterized a novel putative RNA helicase gene, Mj-mov-10 from the kuruma shrimp, Marsupenaeus japonicus and its implication in shrimp RNAi was demonstrated. The full-length Mj-mov-10 gene contained 3536bp, including 239 bp of 5'UTR, 2895 bp of the open reading frame (ORF) and 402bp of 3'UTR, respectively. An ORF of Mj-mov-10 could be translated to a 109-kDa protein which consists of a single helicase core domain containing seven signature motifs of the RNA helicase superfamily-1. Mj-MOV-10 protein shared 47% and 40% identity with mammalian MOV-10 and plant SDE3, respectively. Expression of Mj-mov-10 gene was significantly up-regulated upon dsRNA and white spot syndrome virus (WSSV) challenge. In vivo gene knockdown of Mj-mov-10 resulted in an increase of a susceptibility of shrimp to WSSV infection. Our results implied the functional significance of Mj-MOV-10 in dsRNA-mediated gene silencing and antiviral defense mechanism in shrimp.

• Authors: Amnat Phetrungnapha, Hidehiro Kondo, Ikuo Hirono, Sakol Panyim, Chalermporn Ongvarrasopone
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 44 Issue 1 Pg. 241-7 (May 2015) ISSN: 1095-9947 [Electronic] England
• PMID: 25724627 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Arthropod Proteins
• RNA Helicases

Topics

• Amino Acid Sequence
• Animals
• Arthropod Proteins (genetics)
• Cloning, Molecular
• Molecular Sequence Data
• Penaeidae (genetics)
• RNA Helicases (genetics)
• RNA Interference