Accumulating data indicate translation plays a role in
cancer biology, particularly its rate limiting stage of initiation. Despite this evolving recognition, the function and importance of specific translation
initiation factors is unresolved. The eukaryotic translation initiation complex
eIF4F consists of
eIF4E and
eIF4G at a 1:1 ratio. Although it is expected that they display interdependent functions, several publications suggest independent mechanisms. This study is the first to directly assess the relative contribution of
eIF4F components to the expressed cellular
proteome,
transcription factors,
microRNAs, and phenotype in a
malignancy known for extensive
protein synthesis-
multiple myeloma (MM). Previously, we have shown that
eIF4E/eIF4GI attenuation (
siRNA/
Avastin) deleteriously affected MM cells' fate and reduced levels of
eIF4E/eIF4GI established targets. Here, we demonstrated that
eIF4E/eIF4GI indeed have individual influences on cell
proteome. We used an objective, high throughput assay of
mRNA microarrays to examine the significance of
eIF4E/eIF4GI silencing to several cellular facets such as
transcription factors,
microRNAs and phenotype. We showed different imprints for
eIF4E and eIF4GI in all assayed aspects. These results promote our understanding of the relative contribution and importance of
eIF4E and eIF4GI to the malignant phenotype and shed light on their function in
eIF4F translation initiation complex.