Development of 5Ns chromosome-specific SCAR markers for utilization in future wheat breeding programs.

Abstract

In previous studies, we developed a wheat-Psathyrostachys huashanica Keng disomic addition line 3-8-10-2, which exhibited high stripe rust resistance and could be used as a donor source for introducing novel disease resistance gene(s) into wheat in future breeding programs. It was identified using cytology, genomic in situ hybridization (GISH), EST-SSR, EST-STS and morphological analyses. However, these techniques are not suitable for breeding programs that require the rapid screening of large numbers of genotypes because they are highly technical and time-consuming. In this study, three Ns genome-specific SCAR markers were developed via random amplified polymorphic DNA (RAPD) markers. These SCAR markers were further validated using a complete set of wheat-P. huashanica disomic addition lines, which segregated the 5Ns disomic addition line individuals. Our results indicated that the SCAR markers associated with the 5Ns chromosome of P. huashanica and they provide a low cost, high efficiency, alternative tool for screening 5Ns chromosomes in a wheat background. These newly developed SCAR markers that species-specificity of the markers was proved by analysis of a wide range of cereal species, and specific for 5Ns chromosome, which should be useful in marker-assisted selection for wheat breeders who want to screen genotypes that may contain 5Ns chromatin.

• Authors: J Wang, L M Wang, W L Du, L G Chen, S H Liu, J Wu, J X Zhao, Q H Yang, X H Chen
• Journal: Genetika (Genetika) Vol. 50 Issue 6 Pg. 692-9 (Jun 2014) ISSN: 0016-6758 [Print] Russia (Federation)
• PMID: 25715460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Genetic Markers

Topics

• Breeding (methods)
• Chromosomes, Plant (genetics)
• Genetic Markers
• Random Amplified Polymorphic DNA Technique
• Triticum (genetics)