Human gut microbiota is being increasingly recognized as a player in
colorectal cancers (
CRCs). Evidence suggests that Fusobacterium nucleatum (F. nucleatum) may contribute to
disease progression and is associated with CpG island methylator phenotype (CIMP) and
microsatellite instability (MSI) in
CRCs; however, to date, there are no reports about the relationship between F. nucleatum and molecular features in the early stage of colorectal
tumorigenesis. Therefore, we investigated the presence of F. nucleatum in premalignant colorectal lesions. In total, 465 premalignant lesions (343 serrated lesions and 122 non-serrated
adenomas) and 511
CRCs were studied. We determined the presence of F. nucleatum and analyzed its association with molecular features including CIMP, MSI and microRNA-31 status. F. nucleatum was detected in 24% of hyperplastic
polyps, 35% of sessile serrated
adenomas (SSAs), 30% of traditional serrated
adenomas (TSAs) and 33% of non-serrated
adenomas. F. nucleatum was more frequently detected in CIMP-high premalignant lesions than in CIMP-low/zero lesions (p = 0.0023). In SSAs, F. nucleatum positivity increased gradually from sigmoid colon to cecum (p = 0.042). F. nucleatum positivity was significantly higher in
CRCs (56%) than in premalignant lesions of any histological type (p < 0.0001). In conclusion, F. nucleatum was identified in premalignant colorectal lesions regardless of histopathology but was more frequently associated with CIMP-high lesions. Moreover, F. nucleatum positivity increased according to histological grade, suggesting that it may contribute to the progression of colorectal
neoplasia. Our data also indicate that F. nucleatum positivity in SSAs may support the "colorectal continuum" concept.