Prevalent and up-regulated vimentin expression in micropapillary components of lung adenocarcinomas and its adverse prognostic significance.

Abstract

The factors conferring the increased malignancy on lung adenocarcinoma with micropapillary component (AC-MPC) remain to be elucidated. On proteomics based on 2-dimensional gel electrophoresis, 19 proteins differentially expressed by more than 1.5-fold between AC-MPC and conventional adenocarcinoma (CAC); in particular, vimentin, one of the proteins, was 3.5-fold up-regulated in AC-MPC. Subsequent semi-quantitative investigation by immunohistochemistry with large cohorts comprised 101 AC-MPC and 119 CAC, respectively, of different stages revealed that vimentin was expressed in MPC of 95 (94.1%) AC-MPC and the expression scores were higher than those of well- and moderately differentiated CAC, as well as the background non-MPC of the AC-MPC (P < 0.0001), but not significantly different from those of poorly differentiated CAC (P = 0.561). Even within the AC-MPC entity, higher vimentin expression was correlated with more frequent vascular invasion and more advanced node metastasis (P < 0.02), and multivariate analysis showed that high vimentin expression and worse node statuses were independent indicators of adverse prognosis (P < 0.048). In conclusion, vimentin expression is prevalent and markedly up-regulated in MPC, which might reflect the biological essence of poorer differentiation or dedifferentiation of MPC, and this might have a role in the acquisition and increase of invasiveness and consequent more malignant nature of MPC.

• Authors: Hiroyasu Nakashima, Shi-Xu Jiang, Yuichi Sato, Keika Hoshi, Toshihide Matsumoto, Ryo Nagashio, Makoto Kobayashi, Yukiko Matsuo, Kazu Shiomi, Kazushige Hayakawa, Makoto Saegusa, Yukitoshi Satoh
• Journal: Pathology international (Pathol Int) Vol. 65 Issue 4 Pg. 183-92 (Apr 2015) ISSN: 1440-1827 [Electronic] Australia
• PMID: 25684590 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

Chemical References

• Biomarkers, Tumor
• Vimentin

Topics

• Adenocarcinoma (metabolism, mortality, pathology)
• Adenocarcinoma of Lung
• Aged
• Biomarkers, Tumor (analysis)
• Carcinoma, Papillary (metabolism, mortality, pathology)
• Disease-Free Survival
• Electrophoresis, Gel, Two-Dimensional
• Female
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Lung Neoplasms (metabolism, mortality, pathology)
• Male
• Middle Aged
• Prognosis
• Proportional Hazards Models
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Up-Regulation
• Vimentin (analysis, biosynthesis)