Abstract | BACKGROUND: METHODS: SSV mAb was generated and characterized. Mice were treated with SSV mAb or a control IgG antibody prior to LPS injection. Evaluation of survival rate and lung tissue on histological score was performed. The levels of inflammatory cytokines and histones H1, H3, and H4 in plasma and lung tissue were measured by ELISA. RESULTS: Competitive ELISA revealed that SSV mAb binds to histone H1. SSV mAb improved lung injury and prolonged the survival of LPS-injected mice. Increased levels of histones H1, H3, and H4 and inflammatory cytokines (TNF-α, IL-1β, and IL-6) in plasma and lung tissue after LPS injection were ameliorated by SSV mAb. CONCLUSION: SSV mAb is shown to have anti-inflammatory activity and organ-protective effects, highlighting the importance of controlling histone H1 as well as H3 and H4 levels during inflammation.
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Authors | Toru Kusano, Kuei-Chen Chiang, Masafumi Inomata, Yayoi Shimada, Naoya Ohmori, Takeshi Goto, Shuji Sato, Shigeru Goto, Toshiaki Nakano, Seiji Kawamoto, Yuki Takaoka, Norio Shiraishi, Takayuki Noguchi, Seigo Kitano |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2015
Pg. 491649
( 2015)
ISSN: 2314-6141 [Electronic] United States |
PMID | 25649890
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Cytokines
- Histones
- Lipopolysaccharides
- Peptides
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Topics |
- Animals
- Antibodies, Monoclonal
(pharmacology, therapeutic use)
- Cytokines
(blood)
- Disease Models, Animal
- Histones
(antagonists & inhibitors, immunology)
- Lipopolysaccharides
- Lung
(chemistry, drug effects, pathology)
- Male
- Mice
- Mice, Inbred BALB C
- Peptides
(pharmacology, therapeutic use)
- Pneumonia
(chemically induced, drug therapy, metabolism, mortality)
- Sepsis
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