Lipopeptides extract from Bacillus amyloliquefaciens induce human oral squamous cancer cell death.

Abstract

A lipopeptide extract of Bacillus amyloliquefaciens BACY1 (BLE) was found to induce cell death in human oral squamous cell carcinoma (OSCC) cell lines, SCC4 and SCC25, in this study. The results of MTT assay showed that BLE inhibited OSCC cell proliferation in a dose-dependent manner. BLE was also effective in increasing the sub-G1 phases. Furthermore, when membrane damage in SCC4 cells treated with BLE was monitored by LDH assay, release of LDH was significantly increased. The protein and mRNA levels of pro-apoptotic Bax, and caspase-3 were up-regulated by BLE. Taken together, these results suggest that BLE induces apoptosis and then inhibits the cell proliferation of human OSCC cells.

• Authors: Chen-Hui Kuo, Yun-Wei Lin, Ruey-Shyang Chen
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 16 Issue 1 Pg. 91-6 ( 2015) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25640398 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Lipopeptides
• RNA, Messenger
• bcl-2-Associated X Protein
• L-Lactate Dehydrogenase
• Caspase 3

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Bacillus (metabolism)
• Caspase 3 (genetics, metabolism)
• Cell Line, Tumor
• Cell Membrane (pathology)
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• G1 Phase Cell Cycle Checkpoints (drug effects)
• Humans
• L-Lactate Dehydrogenase (metabolism)
• Lipopeptides (pharmacology)
• Mouth Neoplasms (pathology)
• Neoplasms, Squamous Cell (pathology)
• RNA, Messenger (genetics)
• bcl-2-Associated X Protein (genetics, metabolism)