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In vitro inhibitory effects of terpenoids from Chloranthus multistachys on epithelial-mesenchymal transition via down-regulation of Runx2 activation in human breast cancer.

Abstract
From Chloranthus multistachys, three terpenoids - lupeol (1), henrilabdane B (2), and istanbulin A (3) were isolated. Structures of compounds were established by NMR and MS. We reported here that ISTA (3) suppressed cell invasion, but lupeol (1) and henrilabdane B (2) did not. Furthermore, ISTA significantly inhibited the ability of adhesion and migration in vitro. Next, mechanisms of ISTA-induced inhibitory effects on in vitro metastasis were investigated. Sequential treatment data revealed that ISTA dramatically inhibited EGF-induced EMT. Western blot indicated that ISTA also significantly suppressed expression of E-cadherin, vimentin, and slug. In addition, ISTA inhibited Runx2 activation and phosph-Runx2 expression. Collectively, ISTA exhibited significant inhibitory effects on in vitro metastatic potential via inducing EMT inhibition, which may be associated with inhibition of transcriptional activity of Runx2.
AuthorsJianjiang Fu, Shan Wang, Hong Lu, Junchao Ma, Xiaoqin Ke, Ting Liu, Yongming Luo
JournalPhytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 22 Issue 1 Pg. 165-72 (Jan 15 2015) ISSN: 1618-095X [Electronic] Germany
PMID25636886 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier GmbH. All rights reserved.
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Core Binding Factor Alpha 1 Subunit
  • Diterpenes
  • Pentacyclic Triterpenes
  • RUNX2 protein, human
  • Sesquiterpenes
  • Terpenes
  • istanbulin A
  • lupeol
Topics
  • Antineoplastic Agents, Phytogenic (pharmacology)
  • Breast Neoplasms (metabolism)
  • Cell Line, Tumor
  • Core Binding Factor Alpha 1 Subunit (genetics, metabolism)
  • Diterpenes (pharmacology)
  • Down-Regulation
  • Epithelial-Mesenchymal Transition (drug effects)
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Magnoliopsida (chemistry)
  • Molecular Structure
  • Pentacyclic Triterpenes (pharmacology)
  • Plant Components, Aerial (chemistry)
  • Sesquiterpenes (pharmacology)
  • Terpenes (pharmacology)

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