Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats.

Abstract

Yupingfeng is a Chinese herbal compound used efficaciously to treat respiratory tract diseases. Total glucosides of Yupingfeng have been proven effective in anti-inflammation and immunoregulation. Nevertheless, the role of total extract of Yupingfeng (YTE) in pulmonary fibrosis (PF), a severe lung disease with no substantial therapies, remains unknown. Present study was conducted to elucidate the anti-fibrotic activity of YTE. The rat PF model was induced by intratracheal administration of bleomycin (BLM, 5 mg/kg), and YTE (12 mg/kg/d) was gavaged from the second day. At 14 and 28 days, the lungs were harvested and stained with H&E and Masson's trichrome. The content of hydroxyproline (HYP) and type I collagen (Col-I) were detected, while the protein expression of high-mobility group box 1 (HMGB1), transforming growth factor-beta 1 (TGF-β1), Col-I and α-smooth muscle actin (α-SMA) were analyzed by immunohistochemistry or Western blot. As observed, YTE treatment attenuated the alveolitis and fibrosis induced by BLM, reduced the loss of body weight and increase of lung coefficient. Meanwhile, YTE strongly decreased the levels of HYP and Col-I, and reduced the over-expression of HMGB1, TGF-β1, Col-I and α-SMA. In conclusion, YTE could ameliorate BLM-induced lung fibrosis by alleviating HMGB1 activity and TGF-β1 activation, suggesting therapeutic potential for PF.

• Authors: Liucheng Li, Delin Li, Liang Xu, Ping Zhao, Ziyu Deng, Xiaoting Mo, Ping Li, Lianwen Qi, Jun Li, Jian Gao
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 22 Issue 1 Pg. 111-9 (Jan 15 2015) ISSN: 1618-095X [Electronic] Germany
• PMID: 25636879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier GmbH. All rights reserved.

Chemical References

• Actins
• Collagen Type I
• Drugs, Chinese Herbal
• HMGB1 Protein
• Hbp1 protein, rat
• Transforming Growth Factor beta1
• smooth muscle actin, rat
• yupingfeng
• Bleomycin
• Hydroxyproline

Topics

• Actins (metabolism)
• Animals
• Bleomycin (pharmacology)
• Collagen Type I (metabolism)
• Disease Models, Animal
• Drugs, Chinese Herbal
• Female
• HMGB1 Protein (metabolism)
• Hydroxyproline (metabolism)
• Lung (drug effects, pathology)
• Pulmonary Fibrosis (chemically induced, drug therapy)
• Rats, Sprague-Dawley
• Transforming Growth Factor beta1 (metabolism)