Role of HLA-G1 in trophoblast cell proliferation, adhesion and invasion.
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| Abstract |
Trophoblast cells are important in embryo implantation and fetomaternal tolerance. HLA-G is specifically expressed at the maternal-fetal interface and is a regulator in pregnancy. The aim of the present study was to detect the effect of HLA-G1 on trophoblast cell proliferation, adhesion, and invasion. Human trophoblast cell lines (JAR and HTR-8/SVneo cells) were infected with HLA-G1-expressing lentivirus. After infection, HLA-G1 expression of the cells was detected by western blotting. Cell proliferation was detected by the BrdU assay. The cell cycle and apoptosis of JAR and HTR-8/SVneo cells was measured by flow cytometry (FCM). The invasion of the cells under different conditions was detected by the transwell invasion chamber assay. HLA-G1 didn't show any significant influence on the proliferation, apoptosis, adhesion, and invasion of trophocytes in normal culture conditions. However, HLA-G1 inhibited JAR and HTR-8/SVneo cells invasion induced by hepatocyte growth factor (HGF) under normal oxygen conditions. In conditions of hypoxia, HLA-G1 couldn't inhibit the induction of cell invasion by HGF. HLA-G1 is not an independent factor for regulating the trophocytes. It may play an indirect role in embryo implantation and formation of the placenta.
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| Authors | Feng Jiang, Hongxi Zhao, Li Wang, Xinyu Guo, Xiaohong Wang, Guowu Yin, Yunsheng Hu, Yi Li, Yuanqing Yao |
| Journal | Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 458 Issue 1 Pg. 154-60 (Feb 27 2015) ISSN: 1090-2104 [Electronic] United States |
| PMID | 25634694 (Publication Type: Journal Article) |
| Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
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