Abstract | BACKGROUND: METHODS: The MDA-MB231 human breast carcinoma cell line was exposed to exogenous glycosaminoglycans and changes in cell behavior monitored by western blotting, immunocytochemistry, invasion and collagen degradation assays. Selected receptors including PAR-1 and syndecans were depleted by siRNA treatments to assess cell morphology and behavior. Immunohistochemistry for syndecan-2 and its interacting partner, caveolin-2 was performed on human breast tumor tissue arrays. Two-tailed paired t-test and one-way ANOVA with Tukey's post-hoc test were used in the analysis of data. RESULTS: MDA-MB231 cells were shown to be highly sensitive to exogenous heparan sulfate or heparin, promoting increased spreading, focal adhesion and adherens junction formation with concomitantly reduced invasion and matrix degradation. The molecular basis for this effect was revealed to have two components. First, thrombin inhibition contributed to enhanced cell adhesion and reduced invasion. Second, a specific loss of cell surface syndecan-2 was noted. The ensuing junction formation was dependent on syndecan-4, whose role in promoting actin cytoskeletal organization is known. Syndecan-2 interacts with, and may regulate, caveolin-2. Depletion of either molecule had the same adhesion-promoting influence, along with reduced invasion, confirming a role for this complex in maintaining the invasive phenotype of mammary carcinoma cells. Finally, both syndecan-2 and caveolin-2 were upregulated in tissue arrays from breast cancer patients compared to normal mammary tissue. Moreover their expression levels were correlated in triple negative breast cancers. CONCLUSION:
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Authors | Hooi Ching Lim, Hinke A B Multhaupt, John R Couchman |
Journal | Molecular cancer
(Mol Cancer)
Vol. 14
Pg. 15
(Jan 27 2015)
ISSN: 1476-4598 [Electronic] England |
PMID | 25623282
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Caveolin 2
- Heparan Sulfate Proteoglycans
- Receptor, PAR-1
- Syndecan-4
- Syndecan-2
- Heparin
- Thrombin
- Matrix Metalloproteinase 14
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Topics |
- Breast Neoplasms
(genetics, metabolism, pathology)
- Caveolin 2
(genetics, metabolism)
- Cell Adhesion
- Cell Line, Tumor
- Cell Membrane
(metabolism)
- Extracellular Matrix
(metabolism)
- Female
- Gene Expression
- Heparan Sulfate Proteoglycans
(metabolism)
- Heparin
(metabolism)
- Humans
- Matrix Metalloproteinase 14
(metabolism)
- Neoplasm Invasiveness
- Phenotype
- Receptor, PAR-1
(metabolism)
- Signal Transduction
- Syndecan-2
(genetics, metabolism)
- Syndecan-4
(genetics, metabolism)
- Thrombin
(metabolism)
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