Abstract |
In order to evaluate which of human (h) corticotrophin-like intermediary lobe peptide ( CLIP) or h beta-melanocyte stimulating hormone5-22 (h beta MSH5-22) was the better marker of alternate pro-opiomelanocortin ( POMC) processing, both peptides were simultaneously sought in the same tissue extracts from a normal human pituitary, six corticotrophic adenomas, and four non-pituitary tumours responsible for an ectopic ACTH syndrome. Human CLIP was detected using a combination of gel exclusion chromatography and two different radioimmunoassays (RIAs): a mid- ACTH RIA which recognized ACTH but not CLIP, and a COOH- ACTH RIA which recognized both molecules. Human beta MSH5-22 had been measured previously. Neither hCLIP nor h beta MSH5-22 were detected in the normal or tumoural pituitaries. The four non-pituitary tumours, in contrast, contained both peptides; the hCLIP and h beta MSH5-22 ratios ( CLIP/ CLIP + ACTH and h beta MSH5-22/h beta MSH5-22 + h gamma LPH) ranged from 40 to 94% and from 24 to 46%, respectively. In a given tissue the hCLIP ratio was always higher than the h beta MSH5-22 ratio. hCLIP is therefore the better marker of alternate POMC processing.
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Authors | D Vieau, J F Massias, F Girard, J P Luton, X Bertagna |
Journal | Clinical endocrinology
(Clin Endocrinol (Oxf))
Vol. 31
Issue 6
Pg. 691-700
(Dec 1989)
ISSN: 0300-0664 [Print] England |
PMID | 2560687
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Peptide Fragments
- Corticotropin-Like Intermediate Lobe Peptide
- Pro-Opiomelanocortin
- Adrenocorticotropic Hormone
- Melanocyte-Stimulating Hormones
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Topics |
- ACTH Syndrome, Ectopic
(metabolism)
- Adenoma
(metabolism)
- Adrenocorticotropic Hormone
(analysis)
- Biomarkers, Tumor
(analysis)
- Bronchial Neoplasms
(metabolism)
- Carcinoid Tumor
(metabolism)
- Chromatography, Gel
- Corticotropin-Like Intermediate Lobe Peptide
- Humans
- Melanocyte-Stimulating Hormones
(analysis)
- Paraneoplastic Endocrine Syndromes
(metabolism)
- Peptide Fragments
(analysis)
- Pituitary Neoplasms
(metabolism)
- Pro-Opiomelanocortin
(metabolism)
- Radioimmunoassay
- Thymus Neoplasms
(metabolism)
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