A Hyaluronic Acid-Conjugated Gadolinium Hepatocyte-Specific T1 Contrast Agent for Liver Magnetic Resonance Imaging.

Abstract

PURPOSE:

In this study, we synthesized hyaluronic acid-conjugated gadolinium (HA-diethylene triamine pentaacetic acid (DTPA)-Gd) and evaluated as hepatocyte-specific magnetic resonance imaging (MRI) contrast agent for the diagnosis of hepatic metastasis.

PROCEDURES:

We conducted Fourier transform (FT)-IR analysis to determine the conjugation of HA and DTPA and performed cell viability assays using NIH3T3 and FL83B cell lines. We also conducted T1-weighted MRI of HA-DTPA-Gd and gadoxetic acid to compare the paramagnetic properties of both.

RESULTS:

HA-DTPA-Gd had a higher efficiency in liver MRI compared with the commercially available liver-specific contrast agent (p < 0.001). HA-DTPA-Gd, which possessed a higher T1 relaxivity, showed excellent capability for the diagnosis of hepatic metastasis through an in vivo MRI study in comparison with gadoxetic acid (p < 0.001).

CONCLUSION:

Based on this study, we believe that HA-DTPA-Gd has promising potential for use as a contrast agent for liver MRI of hepatic metastases.

• Authors: MyeongJu Moon, Reju George Thomas, Seon-U Heo, Myong-Suk Park, Woo Kyun Bae, Suk Hee Heo, Nam Yeol Yim, Yong Yeon Jeong
• Journal: Molecular imaging and biology (Mol Imaging Biol) Vol. 17 Issue 4 Pg. 497-503 (Aug 2015) ISSN: 1860-2002 [Electronic] United States
• PMID: 25604386 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Contrast Media
• Hyaluronic Acid
• Gadolinium

Topics

• Animals
• Contrast Media (chemistry, pharmacokinetics)
• Gadolinium (chemistry, pharmacokinetics)
• Hepatocytes (metabolism)
• Hyaluronic Acid (chemistry, pharmacokinetics)
• Liver (metabolism)
• Liver Neoplasms, Experimental (metabolism)
• Magnetic Resonance Imaging (methods)
• Mice
• Mice, Inbred BALB C
• NIH 3T3 Cells