Abstract | BACKGROUND:
Beta lactams are the most commonly used group of antimicrobials worldwide. The presence of extended-spectrum lactamases (ESBL) affects significantly the treatment of infections due to multidrug resistant strains of gram-negative bacilli. The aim of this study was to characterize the beta-lactamase resistance genes in Escherichia coli isolated from nosocomial infections in Cotonou, Benin. METHODS: Escherichia coli strains were isolated from various biological samples such as urine, pus, vaginal swab, sperm, blood, spinal fluid and catheter. Isolated bacteria were submitted to eleven usual antibiotics, using disc diffusion method according to NCCLS criteria, for resistance analysis. Beta-lactamase production was determined by an acidimetric method with benzylpenicillin. Microbiological characterization of ESBL enzymes was done by double disc synergy test and the resistance genes TEM and SHV were screened by specific PCR. RESULTS: CONCLUSION: This study revealed the presence of ESBL producing Eschericiha coli in Cotonou. It demonstrated also high resistance rate to antibiotics commonly used for infections treatment. Continuous monitoring and judicious antibiotic usage are required.
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Authors | Eugénie Anago, Lucie Ayi-Fanou, Casimir D Akpovi, Wilfried B Hounkpe, Micheline Agassounon-Djikpo Tchibozo, Honoré S Bankole, Ambaliou Sanni |
Journal | Annals of clinical microbiology and antimicrobials
(Ann Clin Microbiol Antimicrob)
Vol. 14
Pg. 5
(Jan 17 2015)
ISSN: 1476-0711 [Electronic] England |
PMID | 25595314
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- beta-Lactams
- beta-Lactamases
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Benin
(epidemiology)
- Cross Infection
(epidemiology, microbiology)
- Drug Resistance, Multiple, Bacterial
- Escherichia coli
(drug effects, enzymology, genetics, isolation & purification)
- Escherichia coli Infections
(epidemiology, microbiology)
- Genotype
- Humans
- Microbial Sensitivity Tests
- beta-Lactamases
(genetics, metabolism)
- beta-Lactams
(pharmacology)
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