Synthetic scrapie infectivity: interaction between recombinant PrP and scrapie brain-derived RNA.

Abstract

The key molecular event in human cerebral proteinopathies, which include Alzheimer's, Parkinson's and Huntington's diseases, is the structural conversion of a specific host protein into a β-sheet-rich conformer. With regards to this common mechanism, it appears difficult to explain the outstanding infectious properties attributed to PrP(Sc), the hallmark of another intriguing family of cerebral proteinopathies known as transmissible spongiform encephalopathies (TSE) or prion diseases. The infectious PrP(Sc) or "prion" is thought to be composed solely of a misfolded form of the otherwise harmless cellular prion protein (PrP(c)). To gain insight into this unique situation, we used the 263K scrapie hamster model to search for a putative PrP(Sc)-associated factor that contributes to the infectivity of PrP(Sc) amyloid. In a rigorously controlled set of experiments that included several bioassays, we showed that originally innocuous recombinant prion protein (recPrP) equivalent to PrP(c) is capable of initiating prion disease in hamsters when it is converted to a prion-like conformation (β-sheet-rich) in the presence of RNA purified from scrapie-associated fibril (SAF) preparations. Analysis of the recPrP-RNA infectious mixture reveals the presence of 2 populations of small RNAs of approximately 27 and 55 nucleotides. These unprecedented findings are discussed in light of the distinct relationship that may exist between this RNA material and the 2 biological properties, infectivity and strain features, attributed to prion amyloid.

• Authors: Steve Simoneau, Achim Thomzig, Marie-Madeleine Ruchoux, Nicolas Vignier, Martin L Daus, Anna Poleggi, Pierre Lebon, Sophie Freire, Valerie Durand, Silvia Graziano, Roberta Galeno, Franco Cardone, Emmanuel Comoy, Maurizio Pocchiari, Michael Beekes, Jean-Philippe Deslys, Jean-Guy Fournier
• Journal: Virulence (Virulence) Vol. 6 Issue 2 Pg. 132-44 ( 2015) ISSN: 2150-5608 [Electronic] United States
• PMID: 25585171 (Publication Type: Journal Article)

Chemical References

• Amyloid
• PrPSc Proteins
• Recombinant Proteins
• RNA

Topics

• Amyloid (analysis)
• Animals
• Brain (pathology, ultrastructure)
• Brain Chemistry
• Cricetinae
• Microscopy, Electron
• PrPSc Proteins (analysis, chemistry, genetics, pathogenicity)
• Protein Structure, Secondary
• RNA (analysis, chemistry, genetics, metabolism)
• Recombinant Proteins (chemistry, metabolism)
• Scrapie (etiology)