Abstract | PURPOSE:
Galectin (Gal)-1, a lectin found at sites of immune privilege with critical role in the inflammation, has been poorly investigated in the ocular inflammatory diseases. Here, we evaluated the therapeutic potential of Gal-1 in ocular allergy using a model of ovalbumin (OVA)-induced AC. METHODS: OVA-immunized BALB/c male mice were challenged with eye drops containing OVA on days 14 through 16 with a subset of animals pretreated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex). RESULTS: Recombinant Gal-1 and Dex administration on days 14 through 16 was effective in reducing the clinical signs of allergic conjunctivitis (AC), plasma anti-OVA IgE levels, Th2 (IL-4 and IL-13), and eotaxin/ RANTES levels in the lymph nodes. Four hours after the last OVA challenge, rGal-1 markedly increased Gal-1 endogenous levels in the conjunctiva, and provoked eosinophilia, which persisted at 24 hours. Recombinant Gal-1 had no effect on eosinophil activation, as evidenced by the similar pattern of peroxidase eosinophil expression between cells of rGal-1-treated and untreated AC groups. Conjunctival migrated eosinophils and neutrophils exhibited high levels of Gal-1 and β2-integrin, with points of colocalization, in the rGal-1-treated groups. These different effects observed for rGal-1 were correlated with elevated levels of activated ERK and p38 at 4 hours, and diminished levels of activated JNK and p38 at 24 hours in the eyes. CONCLUSIONS:
Gal-1 has an important role in ocular allergic inflammation and represents a potential target for the development of new therapeutic strategies in eye diseases.
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Authors | Claudia Bosnic Mello, Lisandra Ramos, Alexandre Dantas Gimenes, Teresa Raquel de Moraes Andrade, Sonia Maria Oliani, Cristiane Damas Gil |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 56
Issue 2
Pg. 693-704
(Jan 08 2015)
ISSN: 1552-5783 [Electronic] United States |
PMID | 25574053
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc. |
Chemical References |
- Antibodies, Anti-Idiotypic
- Chemokines
- Galectin 1
- Recombinant Proteins
- Immunoglobulin E
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Topics |
- Animals
- Antibodies, Anti-Idiotypic
(immunology)
- Blotting, Western
- Chemokines
(metabolism)
- Conjunctiva
(drug effects, immunology, ultrastructure)
- Conjunctivitis, Allergic
(drug therapy, immunology, metabolism)
- Disease Models, Animal
- Galectin 1
(pharmacology)
- Immunity, Cellular
- Immunoglobulin E
(immunology)
- Immunohistochemistry
- Immunomodulation
(immunology)
- Male
- Mice
- Mice, Inbred BALB C
- Microscopy, Electron
- Recombinant Proteins
- T-Lymphocytes
(immunology)
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