[Therapeutic study of leukemia by pegylated liposomal daunorubicin].

Abstract

OBJECTIVE:

To explore the antitumor effect and toxicity of pegylated liposomal daunorubicin (PL-DNR) on leukemia.

METHODS:

PL-DNR was prepared by dry lipid hydration and remote loading, and its physicochemical indexes were analyzed. The inhibiting effect of PL-DNR on leukemia cells was observed in terms of in vitro cytotoxicity experiment. The therapeutic effect in vivo was assessed by tumor inhibition in leukemia L1210-bearing mice. Apoptosis in cardiomyocytes was detected using the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method (TUNEL staining).

RESULTS:

The average diameter of PL-DNR was (110 ± 10)nm and the encapsulation efficiency was 94.21%. The in vitro cytotoxicity experiment showed that the inhibiting ability of PL-DNR in the treatment groups was continuously enhanced as the experiment proceeded. The in vivo pharmacodynamic experiment also indicated obvious tumor-inhibiting effect of PL-DNR. At the end of the experiment, the tumor volume of the PL-DNR group was (433.71 ± 234.77)mm(3), significantly smaller than that of (1 293.77 ± 381.26) mm(3) in the DNR group (P < 0.05). Moreover, the tumor weight of the PL-DNR group was (0.66 ± 0.29)g and that of the DNR group was (1.25 ± 0.43)g (P < 0.05). The myocardial toxicity experiment showed that the median apoptosis index of cardiomyocytes in the PL-DNR group was 13.83%, significantly lower than that of 42.67% in the DNR group (P < 0.05), indicating a lower toxicity of PL-DNR to the myocardium.

CONCLUSION:

Compared with the free DNR, PL-DNR can improve the therapeutic effect on leukemia and reduce the.

• Authors: Yongming Zhang, Xinghua Zhang, Wanyan Tang, Jie Ma
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 36 Issue 10 Pg. 746-50 (Oct 2014) ISSN: 0253-3766 [Print] China
• PMID: 25567304 (Publication Type: Journal Article)

Chemical References

• Antibiotics, Antineoplastic
• Daunorubicin

Topics

• Animals
• Antibiotics, Antineoplastic (therapeutic use)
• Apoptosis
• Daunorubicin (therapeutic use)
• Leukemia (therapy)
• Mice