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[Evaluation of a self-prepared anti-WNV-IgG diagnostic ELISA kit with a panel of serum samples collected from the people from areas in which West Nile fever is endemic].

Abstract
In view of that there is no report of west Nile virus infection cases in our country, evaluation the self-prepared anti-WNV-IgG diagnostic ELISA kit should be employed with the establishment of the serum sample panel collected from the entry personnel. All individuals of entry personnel were traveled from epidemic area of infectious west Nile disease. In our study, the serum samples were both detected by self-prepared anti-WNV-IgG diagnostic ELISA kit and the FDA certified kits ,which are FOCUS West Nile Virus IgG Dxselect and Panbio Dengue IgG Capture ELISA kits. The self-prepared kit and FDA certified kits were compared and assessed simultaneously. Furthermore, the specificity, repeatability and stability of the kits were also evaluated. The results indicated that no significant difference of detective rates (35. 6% for self-prepared kit vs. 32.5% for FOCUS kit, χ2 = 3. 05, P > 0.05) and good consistency (Kappa = 0.8372) between the self-prepared kit and FDA certified kits. Also, the positive coincidence rate, the negative coincidence rate and the total coincidence rate were calculated as 91.18%, 95.34% and 92.66%, respectively. The laboratory self-developed kit presented similar quality as the counterpart kits with FDA certificate. The development of our self-prepared anti-WNV-IgG diagnostic ELISA kit will provide technical support for the prevention and control of west Nile virus entry.
AuthorsYu-Chun Wang, Qiang Shao, Li-Ping Zhang, Wei Zhen, Xue-Min Wu, Xue-Zheng Ma, Yong Zhao, Kong-Xin Hu
JournalBing du xue bao = Chinese journal of virology (Bing Du Xue Bao) Vol. 30 Issue 5 Pg. 549-53 (Sep 2014) ISSN: 1000-8721 [Print] China
PMID25562965 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Immunoglobulin G
  • Reagent Kits, Diagnostic
Topics
  • Endemic Diseases
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin G (blood)
  • Reagent Kits, Diagnostic
  • West Nile Fever (epidemiology)
  • West Nile virus (immunology)

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