Differential immuno-reactivity to genomic DNA, RNA and mitochondrial DNA is associated with auto-immunity.

Abstract

BACKGROUND:

Circulating auto-reactive antibodies are hallmark features of auto-immune diseases, however little is known with respect to the specificity of such bio-markers. In the present study, we investigated the specificity of anti-nucleic acid antibodies in the blood of subjects with systemic lupus erythematosus (SLE) and healthy controls.

METHODS:

Sera from 12 SLE cases and 8 controls were evaluated for immuno-reactivity to purified RNA, DNA and mitochondrial DNA (mtDNA) by enzyme-linked immuno-sorbent assay (ELISA).

RESULTS:

As expected, immuno-reactivity to total nucleic acids was significantly higher in subjects with SLE when compared to healthy controls, however a clear distinction was observed among the various nucleic acid sub-types, with sera from SLE subjects displaying the greatest immuno-reactivity to RNA followed by mtDNA and then total DNA.

CONCLUSION:

The identification of auto-reactive antibodies can serve as highly sensitive biomarkers, although their specificity may not always allow diagnostic certainty. The knowledge that auto-antibodies in subjects with SLE display differential immuno-reactivity may help to improve existing diagnostics and may lead to a better understanding of the pathogenesis of auto-immune disorders.

• Authors: Vilena V Ivanova, Svetlana F Khaiboullina, Ekaterina E Cherenkova, Ekaterina V Martynova, Tatiana A Nevzorova, Michail A Kunst, Timur B Sibgatullin, Adelia N Maksudova, Paulo J Oliveira, Vincent C Lombardi, András Palotás, Albert A Rizvanov
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 34 Issue 6 Pg. 2200-8 ( 2014) ISSN: 1421-9778 [Electronic] Germany
• PMID: 25562166 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Mitochondrial
• RNA
• DNA

Topics

• Adolescent
• Adult
• DNA (immunology)
• DNA, Mitochondrial (immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Genome, Human (immunology)
• HEK293 Cells
• Humans
• Lupus Erythematosus, Systemic (immunology, pathology)
• Male
• Mitochondria (immunology)
• RNA (immunology)