Abstract | OBJECTIVE: MATERIALS AND METHODS: After isolation of hearts of male Wister rats, the study was conducted on control and diosgenin- receiving hearts in the presence or absence of 5-HD and L-NAME (as antagonists of mitoKATP channel and NO system, respectively) in an isolated buffer-perfused heart model. Global ischaemia was induced by 30-min occlusion of aortic flow followed by 90-min reperfusion. Cardiac haemodynamics were recorded throughout the experiment using a PowerLab data acquisition system. RESULTS: The levels of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) in the coronary effluents were estimated colourimetrically. Diosgenin pre-administration significantly decreased the release of LDH and CK-MD into the coronary effluent as compared the with the control group (P < 0.05). The left ventricular developed pressure (LVDP) and contractility (± dP/dt) were significantly improved and restored to pre-ischaemic values in the diosgenin-receiving group (P < 0.05). There were no significant differences in left ventricular end-diastolic pressure, coronary flow and heart rate between the control and diosgenin-treated groups during the pre-ischaemic and reperfusion periods. Blocking the mitoKATP channels by 5-HD completely eliminated the positive effect of the diosgenin on the LVDP and ± dP/dt (P < 0.05). However, blocking the NO system by L-NAME slightly reduced the diosgenin effects and the inhibitory effect of L-NAME was less than 5-HD. CONCLUSION:
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Authors | R Badalzadeh, B Yousefi, A Tajaddini, N Ahmadian |
Journal | Perfusion
(Perfusion)
Vol. 30
Issue 7
Pg. 565-71
(Oct 2015)
ISSN: 1477-111X [Electronic] England |
PMID | 25552273
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author(s) 2014. |
Chemical References |
- Potassium Channels
- mitochondrial K(ATP) channel
- Diosgenin
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Topics |
- Animals
- Diosgenin
(pharmacology)
- Male
- Mitochondria, Heart
(metabolism, pathology)
- Myocardial Reperfusion Injury
(prevention & control)
- Potassium Channels
(metabolism)
- Rats
- Rats, Wistar
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