Abstract | BACKGROUND/AIM: Alpha- and beta- amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line. MATERIALS AND METHODS: The alpha- and beta- amanitins used for this research were purified from Amanita phalloides by preparative high-performance liquid chromatography. The MCF-7 breast cancer cell line was used, and specific concentrations of the toxins (100, 10, 1, 0.1, and 0.01 μg/mL) were applied to the cells. The MTT test was performed to determine the level of toxicity, and the quantity of protein in the cell was measured using the biuret test. RESULTS: CONCLUSION:
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Authors | Ertuğrul Kaya, Recep Bayram, Kürşat Oğuz Yaykaşli, İsmail Yilmaz, Sait Bayram, Emine Yaykaşli, Muhsine Zeynep Yavuz, Ali Akçahan Gepdıremen |
Journal | Turkish journal of medical sciences
(Turk J Med Sci)
Vol. 44
Issue 5
Pg. 728-32
( 2014)
ISSN: 1300-0144 [Print] Turkey |
PMID | 25539537
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Alpha-Amanitin
(toxicity)
- Amanitins
(toxicity)
- Humans
- MCF-7 Cells
- Mushroom Poisoning
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