[Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells].

Abstract

OBJECTIVE:

To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells.

METHODS:

We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90α and SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database.

RESULTS:

We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs.

CONCLUSIONS:

There are new mRNA isoforms of HSP90α and SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.

• Authors: Dengpan Lai, Jian Chen, Yani Kang
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 34 Issue 12 Pg. 1738-42 (Dec 2014) ISSN: 1673-4254 [Print] China
• PMID: 25537893 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Cell Line, Tumor
• Cell Transformation, Neoplastic
• Genes, Neoplasm
• Humans
• Polyadenylation
• Stomach Neoplasms (genetics)